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CGH
and FISH is a method for analyzing genomic DNA for unbalanced
genetic alterations. Genomic DNA from the test sample
(e.g., tumor cells) is labeled (e.g., orange/red) and
mixed with normal genomic DNA labeled another color (e.g.,
green) and the mixture is hybridized (FISH) to a normal
human metaphase spread or other reference standard. Regions
of imbalance (increased or decreased copy number) in the
tumor are located or mapped relative to the normal metaphase
chromosomes as increases or decreases in the green to
orange/red fluorescence ratio. |
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