A method of DNA amplification similar to PCR.
LCR differs from PCR because it amplifies the
probe molecule rather than producing amplicon
through polymerization of nucleotides. Two probes
are used per each DNA strand and are ligated together
to form a single probe. LCR uses both a DNA polymerase
enzyme and a DNA ligase enzyme to drive the reaction.
Like PCR, LCR requires a thermal cycler to drive
the rxn and each cycle results in a doubling of
the target nucleic acid molecule. LCR can have
greater specificity than PCR.