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A
powerful method for amplifying specific DNA segments which exploits
certain features of DNA replication. For instance replication requires
a primer and specificity is determined by the sequence and size of
the primer. The method amplifies specific DNA segments by cycles of
template denaturation; primer addition; primer annealing and replication
using thermostable DNA polymerase. The degree of amplification achieved
is set at a theoretical maximum of 2^N, where N is the number of cycles,
eg 20 cycles gives a theoretical 1048576 fold amplification. |
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