| Antibody Information: |
| Antibody | Use at this concentration | Source | Reference | Other info |
| rabbit anti-B-galactosidase serum | IF: 1->1000 | Robert Holmgren | Buenzow and Holmgren (1995) Developmental Biology 170: 338-349. | |
| mouse anti-rat CD2 | Serotec | Batch No:0899 Cat #: MCA154R | ||
| rat anti-Ci monoclonal 1C2 | W: 1->10 | Robert Holmgren | Motzny and Holmgren (1995) Mech Dev. 52(1):137-50. | batch 10/19/98 high background. batch 2/02
has low background. recognizes both Ci-155 and Ci-75 |
| rat anti-Ci monoclonal 2A1 | IF: 1 in 2 W: 1->10 | Robert Holmgren | Motzny and Holmgren (1995) Mech Dev. 52(1):137-50. | recognizes only CI-155 |
| rat anti-Ci monoclonal 2A1 ascites | IF: 1->1000 | Robert Holmgren | Motzny and Holmgren (1995) Mech Dev. 52(1):137-50. | |
| mouse anti-Cos2 monoclonal D6 | W: 0.1 ug/ml IF: 1 ug/ml | David Robbins | concentration is 0.73 ug/ul | |
| mouse anti-Costal-2 (17E11), monoclonal | IF: 1:10 | Dev. Studies Hybridoma Bank (Beachy, P.A.) | Lum et al. (2003) Mol. Cell. 12 (5), 1261-1274 | |
| mouse anti-en/inv monoclonal 4D9 | IF: 1 in 3-4 | Nipam Patel | ||
| mouse anti-en/inv monoclonal 4F11 | IF: 1 in 3-4 | Nipam Patel | Patel, et al. (1989) Cell 58: 955-968. |
Works better on wing discs. This antibody recognizes both the engrailed and invected gene products of Drosophila, but the epitope is not yet precisely mapped. The antibody recognizes the product of engrailed genes in other arthropods including Manduca sexta, grasshopper, and Artmeia, but does not appear to work on vertebrates. |
| mouse anti-eve monoclonal | IF: 1 in 3 | |||
| mouse anti-FLAG M2 | W: 1 ul/ml | SIGMA | CAT# F3165 | |
| mouse anti-Fused (22F10), monoclonal | IF: 1:10 | Dev. Studies Hybridoma Bank (Beachy,P.A.) | Lum et al. (2003) Mol. Cell. 12 (5), 1261-1274 | |
| mouse anti-GFP monoclonal 3E6 | IF: 1->1000 | Molecular Probes | CAT# A-11120 | |
| rabbit anti-GFP polyclonal | IF: 1->1000 | Molecular Probes | CAT# A-11122 | |
| rat anti-gsb-p serum | IF: 1->1000 |
Yu |
||
| rat anti-gsb-d serum | IF: 1->250 | Anne | ||
| mouse anti-HA monoclonal 12CA5 | IF: 1->1000 | Bioreact day 47 (2/27/95) 1.2mg/ml | ||
| rabbit anti-HA polyclonal | W: 1/4000 | Abcam Limited | CAT# ab9110 | |
| mouse anti-lamin | IF: 1 : 10 | Yosef Gruenbaum |
Miller KG et al (1985) Cold Spring Harbor Symp Quant Biol 50: 79-90
|
Bruce Alberts lab |
| mouse anti-myc 9E10 | IF: 1/5 W: 1/5 | Dev. Studies Hybridoma Bank (Bishop, J.M.) | Evan, et al. (1985) Mol Cell Biol. 5: 3610-6. | |
| mouse anti-Phosphotyrosine 4G10 | Upstate | CAT# 05-321 | ||
| mouse anti-ptc monoclonal | IF: 1:50 | I. Guererro | ||
| mouse anti-Smoothened (20C6), monoclonal | IF: 1:10 | Dev. Studies Hybridoma Bank (Beachy, P.A.) | Lum et al. (2003) Mol. Cell. 12 (5), 1261-1274 | |
| anti-Su(fu) monoclonal | W: 0.1 ul/ml | David Robbins | Stegman et al. (2000) JBC, 275: 21809 - 21812 | 0.14 ug/ul The band appears to be ~54 kD, running right between the 67 and 42 kD markers (Prestained, NEB 6-175 kDa) |
| mouse anti-Su(fu) (25H3), monoclonal | IF: 1:10 | Dev. Studies Hybridoma Bank (Beachy, P.A.) | Lum et al. (2003) Mol. Cell. 12 (5), 1261-1274 | |
| mouse anti-wg monoclonal 4D4 | Dev. Studies Hybridoma Bank (Stephen M. Cohen) | Brook, W.J. et al. (1996) Science 273: 1373-1377 | 4D4 | |
| rabbit anti-wg polyclonal | IF: 1->500 | |||