Analytical ultracentrifugation is a powerful biophysical technique for characterizing samples in terms of purity and composition, determining molecular weight , detecting complexes and thier stoichiometries, measuring equilibrium constants for self- and hetero-associating systems. In an AUC experiment the sample is visualized in real time , allowing accurate determination of hydrodynamic and thermodynamic parameters. Our Beckman XLA -70 instrument can measure absorbance in the UV-Vis range. The samples should have absorption between .1 - 1 OD ( in a 1.2 cm pathlength cuvette) at the chosen experimental wavelength.
The Keck Facility has two rotors. The 4-hole rotor holds 3 cells and can be used for sedimentation velocity experiments (2-sector cells , each cell holds one sample (~500 ul volume) and one reference solution) and equilibrium experiments ( 6-sector cells, which hold 3 samples (~150 ul volume each ) and 3 reference solutions). The 8-hole rotor will in general only be used for equilibrium experiments, allowing one to run 21 samples at the same time when using the 6-sector cells (7 cells, each with 3 samples and 3 reference solutions)
AUC Service
Options Offered by the Keck Facility:
Level 1
( Full project) (Experimental Design, Data Acquisition and Data
Analysis)
Level 2
(Experimental Design and Data Acquisition)
Level 3
(Data Acquisition)
Level 4
(Data Analysis)
Level 5
(Self Service – Level 6 training required)
Analytical Ultracentrifgation Training Services:
Level 6
(Instrument Training)
Level 7 (Data analysis : AUC/UltraScan Workshop - offered once a year)
Please contact us for pricing and more information on the AUC services
Location: Cook Hall, 4106
FOM ID: XLA_AUC