Fluorescence Polarization
The Beacon 2000 Variable Temperature Fluorescence Polarization System provides a simple, rapid, and highly sensitive way to study equilibrium binding for a range of biomolecules. It measures binding affinities directly in solution and can be used to study protein-protein, protein-DNA, or receptor-ligand binding and interactions.
- Wavelength range: 360 nm - 700 nm
- Sensitivity (for 500 μL sample):
- Accurate to 10-11 M fluorescein in polarization mode
- Accurate to 10-13 M fluorescein in intensity mode
- Choices for single-tube sample chambers:
- standard chamber: minimum sample volume of 500 μL in disposable 10×75 mm glass test tubes
- low volume sample chamber: minimum sample volume of 100 μL in disposable 6×50 glass test tubes
- Filters
- Configured with fluorescein filters
- External filter holders accessible for use with different fluorophores
- Variable temperature control from 6° - 65° (1° increments)
- Beacon 2000 Data Manager Software:
- Data obtained can be imported into Data Manager software to display and graph data points in real time as they are acquired on the instrument
Location: Cook Hall, 4106
FOM ID: BEACON FLPOL