SEC-MALS-QELS
System components:
Wyatt DAWN HELEOS II – multi-angle (static) light scattering (MALS) detector. The molar mass and root mean square radius of the solute are determined by measuring the intensity of the scattered light - provided that the refractive index increment (dn/dc) of the solute/solvent system is known.
Wyatt Optilab T-rEx - refractometer for measuring the absolute refractive index and its increment
Wyatt QELS - quasi-elastic (dynamic) light scattering (QELS) detector. The diffusion coefficient/hydrodynamic radius can be determined from the temporal fluctuations in the intensity of scattered light
HPLC with size-exclusion columns (SEC), DAD absorbance and fluorescence detectors
In this set-up the sample components are separated according to size and characterized individually by static and dynamic light scattering, absorbance and fluorescence.
Typical applications:
· For
proteins and nucleic acids:
measurements of
molecular weights and radii, oligomerization, heterogeneity and solubility studies,
characterization of macromolecular complexes in terms of size, overall
shape and composition/stoichiomentry
· For synthetic polymers: measurements of molar mass & mass moments, polydispersity, conformation/branching
.
For nanoparticles:
solubility and size distribution measurements and
composition /stoichiometry analysis
FOM ID: MALS_QELS
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