Surface Plasmon Resonance (SPR) System
SPR provides a sensitive probe for molecular interactions by measuring changes in refractive index as an analyte solution flows over a thin layer of a ligand immobilized on a gold sensor chip. The technique does not require labeling and can measure the kinetics and equilibrium of an interaction in one assay. SPR is suitable for a wide range of interactants including proteins, nucleic acids, lipids, carbohydrates, whole cells, and polymers.
The SR7500DC SPR instrument features:
· Dual channel acquisition to provide separate reference and sample channels
· Autosampler holding up to 96 HPLC vials or two 96 or 384 well plates
· Controllable injection volume to conserve precious analytes
· Optional sampling cooling for extended assays
· Interaction temperature controllable between 10°C and 70°C
· Detection of small molecule analytes down to ~100 daltons
· Accessible range of binding affinities from 1 mM to 1 pM
Ligand immobilization can be accomplished using a variety of techniques:
· Amine coupling to carboxylated planar monolayer or branched dextran sensor chips
· Thiol coupling using a native or introduced cysteine
· NeutrAvidin, streptavidin, or avidin capture of biotinylated molecules
· Nickel NTA capture of histidine-tagged molecules
· Protein A capture of antibodies
· Lipid monolayer formation on a hydrophobic surface
Reichert provides an assortment of sensor chips to accommodate different immobilizations:
http://www.reichert.com/products.cfm?pcId=493
FOM ID: SPR