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coimmunoprecipitation
 
Definition:
Biology Glossary search by EverythingBio.com
 
     
  A purification procedure to determine if two different molecules (usually proteins) interact. An antibody specific to the protein of interest is added to a cell lysis. Then the antibody-protein complex is pelleted usually using protein-G sepharose which binds most antibodies. If there are any protein/molecules that bind to the first protein, they will also be pelleted. Identification of proteins in the pellet can be determined by western blot (if an antibody exist) or by sequencing a purified protein band.  
     


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