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A
purification procedure to determine if two different molecules
(usually proteins) interact. An antibody specific to the
protein of interest is added to a cell lysis. Then the
antibody-protein complex is pelleted usually using protein-G
sepharose which binds most antibodies. If there are any
protein/molecules that bind to the first protein, they
will also be pelleted. Identification of proteins in the
pellet can be determined by western blot (if an antibody
exist) or by sequencing a purified protein band. |
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