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SYN:
DNA cloning
The production of a lineage of cells all of which contain one kind
of DNA fragment of interest derived from a population of many kinds
of DNA fragments. Operationally by: inserting (recombining) a population
of DNA molecules, known to contain the DNA of interest, into a population
of vector DNA molecules in such a way that each vector molecule contains
only a single DNA molecule from the original population; transforming
a population of host cells with the vector DNA recombinants such that
each host cell takes up only one vector; growing single host cells
separately (cloning) by plating at low density to form a collection
of separate colonies; screening the colonies (clones) formed for the
presence of the DNA of interest. |
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