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A selection medium for hybrid
cell lines; contains hypoxanthine; aminopterin; thymidine. Only cell
lines expressing both hypoxanthine phosphoribosyl transferase (HPRT+)
and thymidine kinase (TK+) can survive in this medium. Aminopterin
inhibits de novo synthesis of nucleosides, while HPRT and TK supply
them from hypoxanthine and thymidine. |
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