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The
use of antibodies or antisera as histological tools for identifying
patterns of antigen distribution within a tissue or an organism. An
antibody (or mixture of antibodies) that binds to a specific protein
or other antigen is tagged with a fluorescent chemical or an enzyme
that can convert a substrate to a visible dye. The tagged antibody
is incubated with the tissue and after washing unbound antibody away
the bound antibody distribution is revealed by fluorescence microscopy
or incubation with a chromogenic substrate. It is assumed that antibody
distribution reflects antigen distribution. |
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