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A
technique that is used to identify the spatial pattern of expression
of a particular transcript (usually an mRNA). In this technique, the
probe is labeled, either radioactively or by chemically attaching
a fluorochrome visualised by fluorescence or an enzyme that can convert
a substrate to a visible dye. A tissue or organism is soaked in a
solution of single-stranded probe under conditions that allow the
probe to hybridize to complementary RNA sequences in the cells; unhybridized
probe is then removed. Radioactive probe is detected by autoradiography.
Fluorochrome is detected by fluorescence microscopy. Enzyme labeled
probe is detected by soaking the tissue in the substrate; the dye
develops in sites where the transcript of interest was expressed.
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