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Transfer
of electrophoretically separated fragments of DNA, after denaturation,
from the gel to an absorbent sheet of material, such as nitrocellulose,
to which the DNA binds. The sheet is immersed in a solution containing
a labeled probe that will hybridize to fragment(s) of interest. The
method was first devised by E. M. Southern to transfer DNA fragments
from an agarose gel to a nitrocellulose paper for hybridization, but
similar transfer methods are now also used for transfering RNA or
protein to papers of a variety of types followed by hybridization
(RNA) or labeled antibody treatment (protein) to identify specific
molecules. |
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